Difference between revisions of "Part:BBa K1590002"

Revision as of 19:12, 18 September 2015

Human Haptoglobin

Haptoglobin is a human protein with high affinity for haemoglobin. This biobrick is a synthetic gene optimized for expression in E. coli.

Usage and Biology

In normal human blood plasma, haptoglobin circulates and binds to any free haemoglobin released from red blood cells. This is very important in normal physiology since free haemoglobin has potential damaging oxidative activity. The tight haptoglobin-haemoglobin complex can then be removed by the reticuloendothelial system, which is a part of the immune system. Engineered haptoglobin therefore has the potential to bind to, and potentially allow detection of, any free haemoglobin found in the environment.

••••

iGEM Dundee 2015

This synthetic gene was found to produce stable product when expressed in E. coli cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1114
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
+In normal human blood plasma, haptoglobin circulates and binds to any free haemoglobin released from red blood cells. This is very important in normal physiology since free haemoglobin has potential damaging oxidative activity. The tight haptoglobin-haemoglobin complex can then be removed by the reticuloendothelial system, which is a part of the immune system. Engineered haptoglobin therefore has the potential to bind to, and potentially allow detection of, any free haemoglobin found in the environment.
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+<partinfo>BBa_K562000 AddReview 4</partinfo>
+<I>iGEM Dundee 2015</I>
+|width='60%' valign='top'|
+This synthetic gene was found to produce stable product when expressed in <i>E. coli</i> cells.
+|}
+;