Difference between revisions of "Part:BBa K1688000"
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Table 1: Biobricks used for gel electrophoresis, their inserts, restrictions enzymes used for digestion, lengths of inserts and plasmid backbones and expected band lengths.
 
Table 1: Biobricks used for gel electrophoresis, their inserts, restrictions enzymes used for digestion, lengths of inserts and plasmid backbones and expected band lengths.
  
−
Biobrick Code Insert                             Digestion       Insert (bp)Backbone (bp)Expected bands
 
−
BBa_K1688000 Promoter + RBS + Rhl A + RBS + Rhl B EcoRI, PstI 2333 2070         2374, 2037
 
−
BBa_K1688001 RBS + Rhl A + RBS + Rhl B         XbaI, PstI 2333 2070         2324, 2052
 
−
BBa_K1688002 RBS + Rhl A                         EcoRI, PstI 2298 2070         1006, 2037
 
−
BBa_K1688003 RBS + Rhl B                         EcoRI, PstI 1325 2070         1366, 2037
 
  
 
[[File:Example.jpg]]
 
[[File:Example.jpg]]

Revision as of 19:11, 18 September 2015

Mono-rhamnolipid gene (inc RBS, J23101 promoter)

The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (Ba_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688000 is a composite part of a promotor BBa_J23101 and BBa_K1688001 which transcribes the genes RhlA and RhlB into mRNA which eventually leads to translation of proteins that induce mono-rhamnolipid production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 130
    Illegal BamHI site found at 690
    Illegal XhoI site found at 866
    Illegal XhoI site found at 2180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1794
    Illegal NgoMIV site found at 1907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355
    Illegal BsaI site found at 1423
    Illegal BsaI.rc site found at 539

Gel electrophoresis Table 1: Biobricks used for gel electrophoresis, their inserts, restrictions enzymes used for digestion, lengths of inserts and plasmid backbones and expected band lengths.


Example.jpg