Difference between revisions of "Part:BBa K1587000"
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The integrity of our construct was checked by sequencing using VF2 primer.
 
The integrity of our construct was checked by sequencing using VF2 primer.
  
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<center>[[File:TLSE ho1.jpg]]</center>
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<center>[[File:TLSE pcyA ho1.jpg]]</center>
 
<center>'''Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et <i>al.</i> 2009)'''</center>
 
<center>'''Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et <i>al.</i> 2009)'''</center>
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Ho1 is used to catalyse the formation of biliverdin (BV) IXα via heme. It is usully used in association with PcyA enabeling the formation phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:50, 18 September 2015

ho1 with RBS

Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022). ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

TLSE pcyA ho1.jpg
Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et al. 2009)

Ho1 is used to catalyse the formation of biliverdin (BV) IXα via heme. It is usully used in association with PcyA enabeling the formation phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]