Difference between revisions of "Part:BBa K1728000"

Revision as of 18:38, 18 September 2015

IL8 toehold switch RNA sensor

Sequence and Features

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 ===Usage and Biology===
-    Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site(RBS)in the loop. There  are three basic elements of toehold switches including 30bp trigger sequence, bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of oral cancer biomarker IL8(Interleukin 8) mRNA. Once the IL8 mRNA is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.
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