Difference between revisions of "Part:BBa K1728000"

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IL8 toehold switch RNA sensor
 
IL8 toehold switch RNA sensor
    Toehold switch is a secondary structure of RNA. Because of special sequence design, it can form a hairpin structure and hide ribosome binding site(RBS)in the loop. There  are three basic elements of toehold switches including 30bp trigger sequence, bacteria ribosome binding site and linker.The trigger sequence of this part is complementary to a part of oral cancer biomarker IL8(Interleukin 8) mRNA. Once the IL8 mRNA(IL8 partial sequence,BBa_K1728004)is presented, the toehold structure will open. Therefore, the ribosome is able to bind on the RBS and translate the down stream gene.
 
  
 
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Revision as of 18:34, 18 September 2015

IL8 toehold switch RNA sensor

IL8 toehold switch RNA sensor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]