Difference between revisions of "Part:BBa K1714002"
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<partinfo>BBa_K1714002 short</partinfo> | <partinfo>BBa_K1714002 short</partinfo> | ||
| − | + | This part is designed for thanatin expression system regulated by pLac. By adding IPTG, this part express thanatin. However, even we sequenced this part, the sequence was awfully different from that as we expected. In addition, even we subcultured many colonies from LB plate to liquid culture, the number of correctly subcultured products were so few. We thought because of thanatin's strong toxicity, it's very hard to survive with thanatin expression system. So only host cells that lost thanatin expression systems were successfully cultured. So we recommend you not to use this part because this part's sequence may be different from the sequence on this page. | |
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Revision as of 18:33, 18 September 2015
pLac-RBS B0034-thanatin-double terminator
This part is designed for thanatin expression system regulated by pLac. By adding IPTG, this part express thanatin. However, even we sequenced this part, the sequence was awfully different from that as we expected. In addition, even we subcultured many colonies from LB plate to liquid culture, the number of correctly subcultured products were so few. We thought because of thanatin's strong toxicity, it's very hard to survive with thanatin expression system. So only host cells that lost thanatin expression systems were successfully cultured. So we recommend you not to use this part because this part's sequence may be different from the sequence on this page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 233
- 1000COMPATIBLE WITH RFC[1000]