Difference between revisions of "Part:BBa K1714001"
| Line 34: | Line 34: | ||
https://static.igem.org/mediawiki/2015/1/11/Hokkaidou_co-expression_Fig.4.png | https://static.igem.org/mediawiki/2015/1/11/Hokkaidou_co-expression_Fig.4.png | ||
| − | |||
Fig. 8. The growth curve of <i>E. coli</i> including BBa_K1714001 with IPTG or not. | Fig. 8. The growth curve of <i>E. coli</i> including BBa_K1714001 with IPTG or not. | ||
As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity. | As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity. | ||
Revision as of 18:27, 18 September 2015
pLac-RBS B0034-ABF-2-double terminator
This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
In this page, I described the result of the activity assay of ABF-2.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 321
- 1000COMPATIBLE WITH RFC[1000]
In this page, I described the result of the activity assay of ABF-2.
We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not. Activity test for ABF-2 and thanatin →Comparing growth curve between the samples one is induced by IPTG and the other is not induced.
- Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
- Adding 2 µL of IPTG into one and DW into the other.
- Measuring OD600 of these samples once in an hour.
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
Fig. 8. The growth curve of E. coli including BBa_K1714001 with IPTG or not.
As you can see, we can’t say that ABF-2 in BBa_K1714001 has correct antimicrobial activity.