Difference between revisions of "Part:BBa K1713015"
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<partinfo>BBa_K1713015 short</partinfo> | <partinfo>BBa_K1713015 short</partinfo> | ||
| − | + | Expresses Aiia and LuxI proteins. Aiia can hydrolyze AHL internal AHL. LuxI can active the synthetic of AHL. And the expression of LuxI and Aiia are regulated by the concentration of AHL. | |
| + | The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag. | ||
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Latest revision as of 18:21, 18 September 2015
luxpR+B0034+LuxI(-LVA)+B0032+AiiA(-LVA)
Expresses Aiia and LuxI proteins. Aiia can hydrolyze AHL internal AHL. LuxI can active the synthetic of AHL. And the expression of LuxI and Aiia are regulated by the concentration of AHL. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 850
- 1000COMPATIBLE WITH RFC[1000]