Difference between revisions of "Part:BBa K1723002:Experience"
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<h3>This chart show the promoter PAM rich URS J23117 (BBa_K1723001) strength in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723002-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) strength stay low. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3>
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<h3>This chart show the response of the promoter PAM rich URS J23117 (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723002-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3>
 
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<p>promoters strength were assessed using GFPmut2 reporter.</p>  
 
<p>promoters strength were assessed using GFPmut2 reporter.</p>  

Revision as of 18:11, 18 September 2015


Applications of BBa_K1723002




EPF_Lausanne_transistor.png

This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of BBa_K1723000 on BBa_K1723001 promoter

EPF_Lausanne_RESULTS_EXPERIENCE.png

This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:

[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting

In comparison with:

[B] apo-dCas9-ω (=no sgRNA)
[Af] no dCas9-ω, no sgRNA


This chart show the response of the promoter PAM rich URS J23117 (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723002-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne



promoters strength were assessed using GFPmut2 reporter.



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