Difference between revisions of "Part:BBa K1723002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [2] and the specific sequence for the gRNA Z0 was designed from the sequence of the PAM rich URS J23117 promoter of the plasmid pWJ89 D.Bikard [1] sent us. | + | This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [2] and the specific sequence for the gRNA Z0 was designed from the sequence of the PAM rich URS J23117 promoter (BBa_K1723001) of the plasmid pWJ89 D.Bikard [1] sent us. |
===Source=== | ===Source=== | ||
Latest revision as of 17:55, 18 September 2015
sgRNA Z0 expressing cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 179
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 126
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [2] and the specific sequence for the gRNA Z0 was designed from the sequence of the PAM rich URS J23117 promoter (BBa_K1723001) of the plasmid pWJ89 D.Bikard [1] sent us.
Source
This sequence was fully synthesized.
References
[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.
[2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763.