Difference between revisions of "Part:BBa K1675000"

Revision as of 17:47, 18 September 2015

NhaA; sodion-proton antiporter; membrane protein

The gene, NhaA, is the archetypal sodium/proton anti-porter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Lithium-ion concentration. NhaA catalyzes the exchange of 2 protons per soduim ion. The anti-porter active at alkaline pH, whose activity is strongly down-regulated below pH 6.5. Therefore, NhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need in our project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 261
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 458
Illegal BamHI site found at 995
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 321
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1675000 short</partinfo>
-The gene, ''NhaA'', is the archetypal sodium/proton anti-porter in ''E. coli''. It is the only member of the family that is absolutely required by ''E. coli'' for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic  Lithium-ion concentration.
+The gene, ''NhaA'', is the archetypal sodium/proton anti-porter in ''E. coli''. It is the only member of the family that is absolutely required by ''E. coli'' for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Lithium-ion concentration.
-''NhaA'' catalyzes the exchange of 2 protons per sodium ion. Therefore, ''NhaA'' could make sure ''E. coli''’s survival under alkaline pH, which are in desperate need in our project.
+''NhaA'' catalyzes the exchange of 2 protons per soduim ion. The anti-porter active at alkaline pH, whose activity is strongly down-regulated below pH 6.5. Therefore, ''NhaA'' could make sure ''E. coli''’s survival under alkaline pH, which are in desperate need in our project.