Difference between revisions of "Part:BBa K1602009"
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         <h1><small>D</small>-Xylose dehydrogenase - xylB</h1>
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         <h1><small>D</small>-Xylose dehydrogenase xylB</h1>
 
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             <colgroup width=50% span="2">
 
             <colgroup width=50% span="2">
 
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                 <b>xylB</b> is coding for a NAD<sup>+</sup> <small>D</small>-xylose dehydrogenase from <i>Caulobacter crescentus</i>. It  
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                 <i>xylB</i> is coding for a NAD<sup>+</sup> <small>D</small>-xylose dehydrogenase from <i>Caulobacter crescentus</i>. It  
 
                 catalyzes the reaction of <small>D</small>-xylose to <small>D</small>-xylonolactone. The sequence
 
                 catalyzes the reaction of <small>D</small>-xylose to <small>D</small>-xylonolactone. The sequence
 
                 is codon optimized for the usage in <i>E.coli</i>.
 
                 is codon optimized for the usage in <i>E.coli</i>.

Revision as of 17:35, 18 September 2015

D-Xylose dehydrogenase xylB

xylB is coding for a NAD+ D-xylose dehydrogenase from Caulobacter crescentus. It catalyzes the reaction of D-xylose to D-xylonolactone. The sequence is codon optimized for the usage in E.coli.
https://static.igem.org/mediawiki/parts/0/03/BBa_K1602009_image_1.png

Characteristics


Molecular Weight 26641.48
Residues 248
Charge -4.0
Isoelectric Point 5.1521
A280 Molar Extinction Coefficients 36440 (reduced) 36690 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]