Difference between revisions of "Part:BBa K1713004:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1713004 short</partinfo>
 
<partinfo>BBa_K1713004 short</partinfo>
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===Design Notes===
 
===Design Notes===
Planning
+
N/A
 
+
 
+
  
 
===Source===
 
===Source===
  
Planning
+
The promoter was standardized based on a earliest paper of 1997 while other parts were all from iGEM distribution.
  
 
===References===
 
===References===
 +
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.

Latest revision as of 17:35, 18 September 2015

Plac/ara-1+strong RBS+mRFP+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A

Source

The promoter was standardized based on a earliest paper of 1997 while other parts were all from iGEM distribution.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.