Difference between revisions of "Part:BBa K1713004"

 
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<partinfo>BBa_K1713004 short</partinfo>
 
<partinfo>BBa_K1713004 short</partinfo>
  
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Planning
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The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that mRFP produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).
  
 
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Revision as of 17:30, 18 September 2015

Plac/ara-1+strong RBS+mRFP+double terminator

The hybrid promoter ( Plac/ara-1 ) can be activated by the AraC protein in the presence of arabinose and so that mRFP produced. And then the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]