Difference between revisions of "Part:BBa K1709000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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Biobrick BBa_C0061 still contained a Barcode sequence which was deleted.
 
Biobrick BBa_C0061 still contained a Barcode sequence which was deleted.
 
For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
 
For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
−
gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present.
 
  
 
===Source===
 
===Source===

Latest revision as of 17:25, 18 September 2015


LuxI-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.

Source

Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.

References