Difference between revisions of "Part:BBa J63006"
(→‎Promoter characterization)
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===Promoter characterization===
 
===Promoter characterization===
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Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the [Part:BBa_J63006:Experience|Experience page] for details.
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Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the [[Part:BBa_J63006:Experience|Experience page]] for details.
  
 
===Promoter Strength===
 
===Promoter Strength===

Revision as of 17:15, 18 September 2015

yeast GAL1 promoter

GAL1 promoter from S. cerevisiae Contains Kozak sequence, part BBa_J63003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Promoter characterization

Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the Experience page for details.

Promoter Strength

JHU Igem 2011 is working on further characterization of this part through using GFP expression within the next month.


Promoter dose =

Gal1 prevents growth in media containing galactose.

SCUT iGEM 2014 have designed a experiment to quantify Gal1 dose titer.


Figure 1.This year we have .