Difference between revisions of "Part:BBa K1688002"

Revision as of 16:54, 18 September 2015

RhlA Gene

The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomona aeruginosa. The biobrick BBa_K1688002 is an assembly of RBS with BBa_K1331001.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 87
Illegal BamHI site found at 647
Illegal XhoI site found at 823
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 312
Illegal BsaI.rc site found at 496

Revision as of 17:11, 15 September 2015 (view source) Sangeethabupalan (Talk \| contribs) ← Older edit		Revision as of 16:54, 18 September 2015 (view source) Kiira (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1688002 short</partinfo>		<partinfo>BBa_K1688002 short</partinfo>

−	The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from ~~pseudomona~~ aeruginosa. The biobrick BBa_K1688002 is an assembly of RBS with BBa_K1331001.	+	The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The RhlA gene codes for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from ''Pseudomona aeruginosa''. The biobrick BBa_K1688002 is an assembly of RBS with BBa_K1331001.