Difference between revisions of "Part:BBa K1688000"

Revision as of 16:49, 18 September 2015

Mono-rhamnolipid gene (inc RBS, J23101 promoter)

The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (Ba_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688000 is a composite part of a promotor BBa_J23101 and BBa_K1688001 which transcribes the genes RhlA and RhlB into mRNA which eventually leads to translation of proteins that induce mono-rhamnolipid production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 130
Illegal BamHI site found at 690
Illegal XhoI site found at 866
Illegal XhoI site found at 2180
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1794
Illegal NgoMIV site found at 1907
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 355
Illegal BsaI site found at 1423
Illegal BsaI.rc site found at 539

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 __NOTOC__
 <partinfo>BBa_K1688000 short</partinfo>
-The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene Ba_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688000 is a composite part of a promotor BBa_J23101 and BBa_K1688001 which transcribes the genes RhlA and RhlB into mRNA which eventually leads to translation of proteins that induce mono-rhamnolipid production.
+The genes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (Ba_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688000 is a composite part of a promotor BBa_J23101 and BBa_K1688001 which transcribes the genes RhlA and RhlB into mRNA which eventually leads to translation of proteins that induce mono-rhamnolipid production.