Difference between revisions of "Part:BBa K1714001:Experience"
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<li>Measuring OD<sub>600</sub> of these samples once in an hour. </li> | <li>Measuring OD<sub>600</sub> of these samples once in an hour. </li> | ||
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| + | <p>As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:48, 18 September 2015
This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.
In this page, I described the result of the activity assay of ABF-2.
Applications of BBa_K1714001
We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.
Activity test for ABF-2 and thanatin
→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.
- Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
- Adding 2 µL of IPTG into one and DW into the other.
- Measuring OD600 of these samples once in an hour.
As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.
User Reviews
UNIQa2dbffc15d1247a8-partinfo-00000000-QINU UNIQa2dbffc15d1247a8-partinfo-00000001-QINU