Difference between revisions of "Part:BBa K1819004"
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This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter  (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).
 
This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter  (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).
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You can see some results on iGEM team Brasil-USP's wiki page using a Lactose promoter to control GFP and RFP expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:45, 18 September 2015

GFP+TetR+pTetR+mRFP1

This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).

You can see some results on iGEM team Brasil-USP's wiki page using a Lactose promoter to control GFP and RFP expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2211
    Illegal AgeI site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644