Difference between revisions of "Part:BBa K1723000:Experience"
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https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png | https://static.igem.org/mediawiki/2015/9/97/EPF_Lausanne_RESULTS_EXPERIENCE.png | ||
| − | <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/> | + | <p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:<br/><br/> |
| + | |||
[A] sgRNA Z4 (BBa_K1723003), activating<br/> | [A] sgRNA Z4 (BBa_K1723003), activating<br/> | ||
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting<br/> | [A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting<br/> | ||
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting<br/> | [A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting<br/> | ||
[R2] sgRNA Z0 (BBa_K1723002), inhibiting<br/> | [R2] sgRNA Z0 (BBa_K1723002), inhibiting<br/> | ||
| − | [R1] sgRNA Z35 (BBa_K1723004), inhibiting<br/> | + | [R1] sgRNA Z35 (BBa_K1723004), inhibiting<br/> <br/> |
| − | In comparison with:<br/> | + | |
| − | [B] <br/> | + | In comparison with:<br/> <br/> |
| − | [Af]<br/> | + | |
| + | [B] The basal level, apo-dCas9-ω (no sgRNA) <br/> | ||
| + | [Af]The auto fluorescence level, no dCas9-ω<br/> | ||
Revision as of 16:44, 18 September 2015
Applications of BBa_K1723000
This picture represent the system we built using dCas9-ω : a transistor-like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117 promoter (BBa_K1723001) when provided following sgRNAs:
[A] sgRNA Z4 (BBa_K1723003), activating
[A/R1] sgRNA Z4 (BBa_K1723003), activating, AND sgRNA Z35 (BBa_K1723004), inhibiting
[A/R2] sgRNA Z4 (BBa_K1723003), activating AND sgRNA Z0 (BBa_K1723002), inhibiting
[R2] sgRNA Z0 (BBa_K1723002), inhibiting
[R1] sgRNA Z35 (BBa_K1723004), inhibiting
In comparison with:
[B] The basal level, apo-dCas9-ω (no sgRNA)
[Af]The auto fluorescence level, no dCas9-ω
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