Difference between revisions of "Part:BBa K1675010"

Revision as of 16:37, 18 September 2015

FimE recombinase device

FimE is different from other kinds of recombinase. Its recognized sites on the upstream (IRL) and downstream (IRR) are not same. It means that after the recombination mediated by FimE happens, the sign sites would be exchanged, so that the new sign sites couldn’t be recognized by FimE again. So the recombination mediated by FimE is irreversible. Granted that the bacteria produce the strong alkali in the primary, the environment would turn alkaline quickly. So P-atp2 can correspond the change of environment and start the expression of FimE. FimE recognizes IRL and IRR, then catalyze the inversion of J23119. The weak acid would be generated at that time. For the environment, due to the production of the weak acid, it would turn neutral gradually.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1675010 short</partinfo>
-Alkali-induced promoter ''P-atp2'' ,ribosome binding site B0034 and recombinase FimE(BBa_K137007)
+FimE is different from other kinds of recombinase. Its recognized sites on the upstream (IRL) and downstream (IRR) are not same. It means that after the recombination mediated by FimE happens, the sign sites would be exchanged, so that the new sign sites couldn’t be recognized by FimE again. So the recombination mediated by FimE is irreversible.
-The part would express recombinase FimE under the alkali environment.
+Granted that the bacteria produce the strong alkali in the primary, the environment would turn alkaline quickly. So P-atp2 can correspond the change of environment and start the expression of FimE. FimE recognizes IRL and IRR, then catalyze the inversion of J23119. The weak acid would be generated at that time. For the environment, due to the production of the weak acid, it would turn neutral gradually.
 <!-- Add more about the biology of this part here