Difference between revisions of "Part:BBa K1758311:Design"
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The original source of our chromate responsive promoter is <i>Ochrobactrum tritici</i> 5bvl1. We used a codon optimized version which we got synthesised by IDT. | The original source of our chromate responsive promoter is <i>Ochrobactrum tritici</i> 5bvl1. We used a codon optimized version which we got synthesised by IDT. |
Revision as of 16:37, 18 September 2015
_NOTOC__ Fumarate reductase (frd) from E. coli under the control of the T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 666
Illegal AgeI site found at 741
Illegal AgeI site found at 3023 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
===Source
The original source of our chromate responsive promoter is Ochrobactrum tritici 5bvl1. We used a codon optimized version which we got synthesised by IDT.