Difference between revisions of "Part:BBa K1714001:Experience"

Revision as of 16:30, 18 September 2015

This part will be finally introduced upstream of BBa_K1714004 (P_BAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.

In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

Diluting the bacterial culture which is enough pre-cultured by 0.1 OD₆₀₀ (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
Adding 2 µL of IPTG into one and DW into the other.
Measuring OD₆₀₀ of these samples once in an hour.

UNIQ0eaab7772ab25f4a-partinfo-00000000-QINU UNIQ0eaab7772ab25f4a-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+<p>This part will be finally introduced upstream of BBa_K1714004 (P<sub>BAD</sub>-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system.</p>
-how you used this part and how it worked out.
+<p>In this page, I described the result of the activity assay of ABF-2.</p>
 ===Applications of BBa_K1714001===