Difference between revisions of "Part:BBa K1819002"
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==TAT signal== | ==TAT signal== | ||
| − | Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of ''Escherichia coli''. We designed TAT signal based on Yikmis ''et al''<sup>1<sup> paper that describes a TAT signal from HyaA (hydrogenase 1 small subunit) been used to export Lcp . | + | Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of ''Escherichia coli''. We designed TAT signal based on Yikmis ''et al''<sup>1</sup> paper that describes a TAT signal from HyaA (hydrogenase 1 small subunit) been used to export Lcp . |
SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported. | SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported. | ||
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| − | <img src="https://static.igem.org/mediawiki/2015/2/28/Brasil-USPTatsignalscai.png" width= "150"> </img src> | + | <center><img src="https://static.igem.org/mediawiki/2015/2/28/Brasil-USPTatsignalscai.png" width= "150"> </img src> |
| + | <p> Schematic representation of BBa_K1819002 insert.</p></center> | ||
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Revision as of 16:30, 18 September 2015
TAT signal
Twin-arginine translocation signal (TAT signal) can be used to protein secretion to the extracellular environment of Escherichia coli. We designed TAT signal based on Yikmis et al1 paper that describes a TAT signal from HyaA (hydrogenase 1 small subunit) been used to export Lcp .
SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.
Schematic representation of BBa_K1819002 insert.
Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.
References
1. Yikmis, M. et al. Secretion and transcriptional regulation of the latex-clearing protein, lcp, by the rubber-degrading bacterium Streptomyces sp strain K30. Appl. Environ. Microbiol. 74, 5373-5382, doi:10.1128/aem.01001-08 (2008).
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]