Difference between revisions of "Part:BBa K1714001:Experience"

 
(→‎Applications of BBa_K1714001)
Line 5: Line 5:
  
 
===Applications of BBa_K1714001===
 
===Applications of BBa_K1714001===
 +
<p>We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.</p>
 +
<p>Activity test for ABF-2 and thanatin</p>
 +
<p>→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.</p>
 +
  <ol>
 +
  <li>Diluting the bacterial culture which is enough pre-cultured by 0.1 OD<sub>600</sub> (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol) </li>
 +
  <li>Adding 2 µL of IPTG into one and DW into the other.</li>
 +
  <li>Measuring OD<sub>600</sub> of these samples once in an hour. </li>
 +
  </ol>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:24, 18 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1714001

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

User Reviews

UNIQ3d173e0c2f88e172-partinfo-00000000-QINU UNIQ3d173e0c2f88e172-partinfo-00000001-QINU