Difference between revisions of "Part:BBa K1789017"
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===Colony PCR Test=== | ===Colony PCR Test=== | ||
| − | [[File:GFP P.jpg| | + | [[File:GFP P.jpg|700px|]] |
Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system. | Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system. | ||
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The plasmid was transformed into E.coli[BL21], and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence. | The plasmid was transformed into E.coli[BL21], and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence. | ||
| − | [[File:GFP_P_F.jpg| | + | [[File:GFP_P_F.jpg|400px|]] |
Fig. 2 Picture drawn with bacteria transformed by GFP_P | Fig. 2 Picture drawn with bacteria transformed by GFP_P | ||
Revision as of 16:22, 18 September 2015
GFP_P
This device is a positive control of GFP with scaffold.
Usage and Biology
This part is positive control in GFP series. Use to comparison with other device in GFP series in light emission. In this part, GFP is normally expressed. It proves that we have no problems with our experimental system. And the Plac, RBS30, terminator is all working well.
GFP is a shared protein in biology experiment. We utilize its luminous properties to visually display that scaffold could make two proteins close enough.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 873
Experimental Validation
Sequencing
This part is sequenced as correct after construction.
Colony PCR Test
Fig. 1 results of Colony PCR Test of GFP_P. We use pSB1C3-F and pSB1C3-R as the forward primer and reverse primer in this colony PCR system.
Fuctional Test
The plasmid was transformed into E.coli[BL21], and was cultured under the 1mM of IPTG induction overnight. We use it to draw some pictures to test whether it can emit visible fluorescence.
Fig. 2 Picture drawn with bacteria transformed by GFP_P