Difference between revisions of "Part:BBa K1587008:Design"

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===Source===
 
===Source===
  
−
Sequences are from cyanobacteria ''Synechocystis sp.'' PCC 6803 :<br>
+
Sequences from cyanobacteria ''Synechocystis sp.'' PCC 6803 :<br>
 
- Cph1 domain<br>
 
- Cph1 domain<br>
 
- Ho1 BBa_I15008<br>
 
- Ho1 BBa_I15008<br>
 
- PcyA BBa_I15009<br>
 
- PcyA BBa_I15009<br>
 +
 +
envZ is a sequence from ''Escherichia coli''.
  
 
===References===
 
===References===

Revision as of 16:13, 18 September 2015

Red light biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No information

Source

Sequences from cyanobacteria Synechocystis sp. PCC 6803 :
- Cph1 domain
- Ho1 BBa_I15008
- PcyA BBa_I15009

envZ is a sequence from Escherichia coli.

References

- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571

- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442

- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable gene expression in Escherichia coli using a miniaturized photobioreactor. PLoS ONE 8: e52382