Difference between revisions of "Part:BBa K1713000:Design"
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===Source=== | ===Source=== | ||
| − | BBa_K094120 | + | It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation. |
===References=== | ===References=== | ||
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210. | Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210. | ||
Latest revision as of 16:07, 18 September 2015
Plac/ara-1, a hybrid promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 28
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.
Source
It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.
References
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.