Difference between revisions of "Part:BBa J31007"
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<partinfo>BBa_J31007 short</partinfo>
 
<partinfo>BBa_J31007 short</partinfo>
  
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BBa_J31007 is the coding region of the Tet resistance gene. It requires a promoter and RBS for the expression of tetracyline resistance.
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BBa_J31007 is the coding region for tetracyline resistance [TetA(C) inner membrane associated protein] in the forward orientation. This does not code for the repressor of the tetracycline promoter. This part requires a promoter and RBS for proper expression.  
  
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===Usage and Biology===
 
===Usage and Biology===
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Cells expressing BBa_J31007 are able to grow on media containing tetracycline, however overexpression is  detrimental to cell growth.
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Revision as of 18:51, 22 March 2007

tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]

BBa_J31007 is the coding region for tetracyline resistance [TetA(C) inner membrane associated protein] in the forward orientation. This does not code for the repressor of the tetracycline promoter. This part requires a promoter and RBS for proper expression.

Usage and Biology

Cells expressing BBa_J31007 are able to grow on media containing tetracycline, however overexpression is detrimental to cell growth.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844
  • 1000
    COMPATIBLE WITH RFC[1000]