Difference between revisions of "Part:BBa K1587007"
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<center>[[File:TLSE ForMetabo.jpg]]</center>
 
<center>[[File:TLSE ForMetabo.jpg]]</center>
 
<center>'''Figure 2: Metabolic pathway to produce formate via glucose using BBa_K1587007 in ''E. coli''.'''</center>
 
<center>'''Figure 2: Metabolic pathway to produce formate via glucose using BBa_K1587007 in ''E. coli''.'''</center>
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Formate production increased significantly by 10 % compared to wt with BBa_K1587007 in ''E. coli''.
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<center>[[File:TLSE ForProd.PNG]]</center>
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<center>'''Figure 3: Summary of formate production by NMR analysis after 3 days culture under micro-aerobic condition'''</center>
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 16:04, 18 September 2015

Formate production

Strong RBS (BBa_1006) and formate producing enzyme pyruvate formate-lyase 1 pflB and pyruvate formate-lyase 1-activating enzyme pflA (Figure 1).

TLSE For.jpg
Figure 1: Genetic construction for formate production.

Formate is a simple organic acid produced with an E.coli strain. The initial substrate, glucose, is decomposed into pyruvate during glycolysis, and formate is naturally synthesized thanks to two key genes:
- pflB coding for pyruvate formate lyase which catalyzes the cleavage of pyruvate into C1 and C2. This enzyme is sensitive to oxygen and is only active in microaerobic or anaerobic conditions, which is the case within our device (Figure 2).
- pflA coding for pyruvate formate lyase activase, which is directly linked with the pyruvate formate lyase, enabling its activation (Figure 2).

TLSE ForMetabo.jpg
Figure 2: Metabolic pathway to produce formate via glucose using BBa_K1587007 in E. coli.

Formate production increased significantly by 10 % compared to wt with BBa_K1587007 in E. coli.

TLSE ForProd.PNG
Figure 3: Summary of formate production by NMR analysis after 3 days culture under micro-aerobic condition

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 405
    Illegal BamHI site found at 2807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2995