Difference between revisions of "Part:BBa K1593997"
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After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction.
 
After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction.
  
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Latest revision as of 16:03, 18 September 2015

R0010-RBS-cheZ

This part is used to coding a chemotactci protein,cheZ,downstream of lac promotor.


lac

Information of lac:see BBa_R0010


cheZ

Information of cheZ:see BBa_K1593998


SDS-PAGE

After induced by IPTG in 1mM about 4 h, cheZ was found in SDS-PAGE gel, compared to the wild type (Top10) and bacteria without IPTG induction. Through this result, we concluded that cheZ has been successfully expressed with the IPTG induction.

4905.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 601
    Illegal NgoMIV site found at 736
  • 1000
    COMPATIBLE WITH RFC[1000]