Difference between revisions of "Part:BBa K1682011"
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=NsrR - Nitric oxide sensitive P<sub>yeaR</sub> repressor protein= | =NsrR - Nitric oxide sensitive P<sub>yeaR</sub> repressor protein= | ||
===Biology of NsrR=== | ===Biology of NsrR=== | ||
| − | [[File:HKUST-Rice 2015 | + | [[File:Team HKUST-Rice 2015 Nitrate PyeaR trans.gif|thumb|500px|center|<b>Fig.1 </b>Nitrate sensing mechanism of P<sub>yeaR</sub>]] |
<i>Escherichia coli</i> (<i>E. coli</i>) detects environmental nitrate by the <i>yeaR-yoaG</i> operon. According to Figure 1, P<sub>yeaR</sub> (Lin, <i>et al.</i>, 2007) is regulated by the Nar two-component regulatory system (Nohno <i>et al.</i>, 1989; Li <i>et al.</i>, 1987) and NsrR nitric oxide sensitive repressor protein (Partridge <i>et al.</i>, 2009). When there is nitrate or nitrite, the repression from the Nar system on P<sub>yeaR</sub> will be relieved due to the binding between the two. On the other hand, some nitrate will be converted into nitric oxide by nitrate reductase. Nitric oxide will bind to the NsrR protein and relieve the repression on P<sub>yeaR</sub>. As a result, any genes that are downstream of P<sub>yeaR</sub> will be expressed. | <i>Escherichia coli</i> (<i>E. coli</i>) detects environmental nitrate by the <i>yeaR-yoaG</i> operon. According to Figure 1, P<sub>yeaR</sub> (Lin, <i>et al.</i>, 2007) is regulated by the Nar two-component regulatory system (Nohno <i>et al.</i>, 1989; Li <i>et al.</i>, 1987) and NsrR nitric oxide sensitive repressor protein (Partridge <i>et al.</i>, 2009). When there is nitrate or nitrite, the repression from the Nar system on P<sub>yeaR</sub> will be relieved due to the binding between the two. On the other hand, some nitrate will be converted into nitric oxide by nitrate reductase. Nitric oxide will bind to the NsrR protein and relieve the repression on P<sub>yeaR</sub>. As a result, any genes that are downstream of P<sub>yeaR</sub> will be expressed. | ||
Revision as of 16:02, 18 September 2015
NsrR - Nitric oxide sensitive PyeaR repressor protein
Biology of NsrR
Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. According to Figure 1, PyeaR (Lin, et al., 2007) is regulated by the Nar two-component regulatory system (Nohno et al., 1989; Li et al., 1987) and NsrR nitric oxide sensitive repressor protein (Partridge et al., 2009). When there is nitrate or nitrite, the repression from the Nar system on PyeaR will be relieved due to the binding between the two. On the other hand, some nitrate will be converted into nitric oxide by nitrate reductase. Nitric oxide will bind to the NsrR protein and relieve the repression on PyeaR. As a result, any genes that are downstream of PyeaR will be expressed.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 154
- 1000COMPATIBLE WITH RFC[1000]