Difference between revisions of "Part:BBa K1797011"

Latest revision as of 16:01, 18 September 2015

RecA

RecA is an essential protein for DNA repair and maintenance. Researchers (Stahl F W, Stahl M M, Malone R E. Red-mediated recombination of phage lambda in a recA− recB− host[J]. Molecular and General Genetics MGG, 1978, 159(2): 207-211.) have found that red recombination system works better in recA+ strains. To maximize the efficiency of red recombination system, we need to introduce recA exogenously in plasmid form though recA is intrinsically existent in E.coli. To have a clearer view of red recombination system, you can refer to BBa_K1797014 and BBa_K1797013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 127
Illegal AgeI site found at 631
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1797011 short</partinfo>
-RecA is a protein that can carry out recombination reactions in E.coli. For example, if you want to disrupt a certain gene's function, you can design a linear DNA fragment with sequences that are homologous to the target gene on both ends. When RecA and the DNA fragment are introduced into E.coli together, homologous recombination takes place.
+RecA is an essential protein for DNA repair and maintenance. Researchers (Stahl F W, Stahl M M, Malone R E. Red-mediated recombination of phage lambda in a recA− recB− host[J]. Molecular and General Genetics MGG, 1978, 159(2): 207-211.) have found that red recombination system works better in recA+ strains. To maximize the efficiency of red recombination system, we need to introduce recA exogenously in plasmid form though recA is intrinsically existent in E.coli. To have a clearer view of red recombination system, you can refer to BBa_K1797014 and BBa_K1797013.
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