Difference between revisions of "Part:BBa K1587009"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1587009 short</partinfo>
 
<partinfo>BBa_K1587009 short</partinfo>
  
−
Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes (formate/butyrate).
+
Uses a positively regulated promoter (P<sub>ompC</sub>) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by Cph8 red light receptor (Cph1-EnvZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes (formate/butyrate).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:57, 18 September 2015

PompC and lacI box regulated cI and formate production

Uses a positively regulated promoter (PompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by Cph8 red light receptor (Cph1-EnvZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes (formate/butyrate).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3908