Difference between revisions of "Part:BBa K1725301"
Line 11: Line 11:
 
|-
 
|-
 
| Degenerate sequence
 
| Degenerate sequence
−
| row 1, cell 2
+
| TCACACA<font color="red">NR</font>A<font color="red">RR</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725301
 
| BBa_K1725301
−
| row 1, cell 2
+
| TCACACA<font color="red">GG</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725302
 
| BBa_K1725302
−
| row 2, cell 2
+
| TCACACA<font color="red">GG</font>A<font color="red">GA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725303
 
| BBa_K1725303
−
| row 1, cell 2
+
| TCACACA<font color="red">GG</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725304
 
| BBa_K1725304
−
| row 1, cell 2
+
| TCACACA<font color="red">AG</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725305
 
| BBa_K1725305
−
| row 2, cell 2
+
| TCACACA<font color="red">AG</font>A<font color="red">AG</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725306
 
| BBa_K1725306
−
| row 1, cell 2
+
| TCACACA<font color="red">CG</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725307
 
| BBa_K1725307
−
| row 1, cell 2
+
| TCACACA<font color="red">TG</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725308
 
| BBa_K1725308
−
| row 2, cell 2
+
| TCACACA<font color="red">AA</font>A<font color="red">AG</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725309
 
| BBa_K1725309
−
| row 1, cell 2
+
| TCACACA<font color="red">AA</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725310
 
| BBa_K1725310
−
| row 1, cell 2
+
| TCACACA<font color="red">CA</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725311
 
| BBa_K1725311
−
| row 2, cell 2
+
| TCACACA<font color="red">CA</font>A<font color="red">GG</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725312
 
| BBa_K1725312
−
| row 1, cell 2
+
| TCACACA<font color="red">GA</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725313
 
| BBa_K1725313
−
| row 1, cell 2
+
| TCACACA<font color="red">TA</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725314
 
| BBa_K1725314
−
| row 2, cell 2
+
| TCACACA<font color="red">TA</font>A<font color="red">GG</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725315
 
| BBa_K1725315
−
| row 1, cell 2
+
| TCACACA<font color="red">TG</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725316
 
| BBa_K1725316
−
| row 1, cell 2
+
| TCACACA<font color="red">CG</font>A<font color="red">AG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725317
 
| BBa_K1725317
−
| row 2, cell 2
+
| TCACACA<font color="red">GG</font>A<font color="red">AA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725318
 
| BBa_K1725318
−
| row 1, cell 2
+
| TCACACA<font color="red">AG</font>A<font color="red">GA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725319
 
| BBa_K1725319
−
| row 1, cell 2
+
| TCACACA<font color="red">TG</font>A<font color="red">GA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725320
 
| BBa_K1725320
−
| row 2, cell 2
+
| TCACACA<font color="red">CG</font>A<font color="red">GA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725321
 
| BBa_K1725321
−
| row 1, cell 2
+
| TCACACA<font color="red">GA</font>A<font color="red">GG</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725322
 
| BBa_K1725322
−
| row 1, cell 2
+
| TCACACA<font color="red">AA</font>A<font color="red">GA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725323
 
| BBa_K1725323
−
| row 2, cell 2
+
| TCACACA<font color="red">CA</font>A<font color="red">GA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725324
 
| BBa_K1725324
−
| row 1, cell 2
+
| TCACACA<font color="red">GA</font>A<font color="red">GA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725325
 
| BBa_K1725325
−
| row 1, cell 2
+
| TCACACA<font color="red">TA</font>A<font color="red">GA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725326
 
| BBa_K1725326
−
| row 2, cell 2
+
| TCACACA<font color="red">AG</font>A<font color="red">AA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725327
 
| BBa_K1725327
−
| row 1, cell 2
+
| TCACACA<font color="red">AA</font>A<font color="red">AA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725328
 
| BBa_K1725328
−
| row 1, cell 2
+
| TCACACA<font color="red">CA</font>A<font color="red">AA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725329
 
| BBa_K1725329
−
| row 2, cell 2
+
| TCACACA<font color="red">GA</font>A<font color="red">AA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|-
 
|-
 
| BBa_K1725330
 
| BBa_K1725330
−
| row 1, cell 2
+
| TCACACA<font color="red">TA</font>A<font color="red">AA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725331
 
| BBa_K1725331
−
| row 1, cell 2
+
| TCACACA<font color="red">TG</font>A<font color="red">AA</font>G
 
| row 1, cell 3
 
| row 1, cell 3
 
|-
 
|-
 
| BBa_K1725332
 
| BBa_K1725332
−
| row 2, cell 2
+
| TCACACA<font color="red">CG</font>A<font color="red">AA</font>G
 
| row 2, cell 3
 
| row 2, cell 3
 
|}
 
|}

Revision as of 15:39, 18 September 2015

Member of the RBS library - derived from BBa_B0032

Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR.

Identifier Sequence Estimated strength (au)
Degenerate sequence TCACACANRARRG row 1, cell 3
BBa_K1725301 TCACACAGGAGGG row 1, cell 3
BBa_K1725302 TCACACAGGAGAG row 2, cell 3
BBa_K1725303 TCACACAGGAAGG row 1, cell 3
BBa_K1725304 TCACACAAGAGGG row 1, cell 3
BBa_K1725305 TCACACAAGAAGG row 2, cell 3
BBa_K1725306 TCACACACGAGGG row 1, cell 3
BBa_K1725307 TCACACATGAGGG row 1, cell 3
BBa_K1725308 TCACACAAAAAGG row 2, cell 3
BBa_K1725309 TCACACAAAAGGG row 1, cell 3
BBa_K1725310 TCACACACAAAGG row 1, cell 3
BBa_K1725311 TCACACACAAGGG row 2, cell 3
BBa_K1725312 TCACACAGAAAGG row 1, cell 3
BBa_K1725313 TCACACATAAAGG row 1, cell 3
BBa_K1725314 TCACACATAAGGG row 2, cell 3
BBa_K1725315 TCACACATGAAGG row 1, cell 3
BBa_K1725316 TCACACACGAAGG row 1, cell 3
BBa_K1725317 TCACACAGGAAAG row 2, cell 3
BBa_K1725318 TCACACAAGAGAG row 1, cell 3
BBa_K1725319 TCACACATGAGAG row 1, cell 3
BBa_K1725320 TCACACACGAGAG row 2, cell 3
BBa_K1725321 TCACACAGAAGGG row 1, cell 3
BBa_K1725322 TCACACAAAAGAG row 1, cell 3
BBa_K1725323 TCACACACAAGAG row 2, cell 3
BBa_K1725324 TCACACAGAAGAG row 1, cell 3
BBa_K1725325 TCACACATAAGAG row 1, cell 3
BBa_K1725326 TCACACAAGAAAG row 2, cell 3
BBa_K1725327 TCACACAAAAAAG row 1, cell 3
BBa_K1725328 TCACACACAAAAG row 1, cell 3
BBa_K1725329 TCACACAGAAAAG row 2, cell 3
BBa_K1725330 TCACACATAAAAG row 1, cell 3
BBa_K1725331 TCACACATGAAAG row 1, cell 3
BBa_K1725332 TCACACACGAAAG row 2, cell 3


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]