Difference between revisions of "Part:BBa K1797010"

Latest revision as of 15:33, 18 September 2015

Kana cassette flanked by loxP site

This part includes a translational kanamycin resistance gene(in prokaryotes) or a neomycin resistance gene(in eukaryotes) and flanking loxP sites, which can be recognized by Cre recombinase. We can use DNA recombination technology to knock this part into E.coli genome and the antibiotic resistance gene helps us find the right colonies. Then if Cre is expressed in the bacteria, this kana cassette can be excised and a knock out experiment is completed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 367
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1031
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 880
Illegal SapI.rc site found at 1090

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 __NOTOC__
 <partinfo>BBa_K1797010 short</partinfo>
-This part includes a translational kanamycin resistance gene(in prokaryotes) or a neomycin resistance gene(in eukaryotes) and flanking loxP sites. We can use DNA recombination technology to knock this part into E.coli genome and the antibiotic resistance gene helps us find the right colonies. Then if Cre is expressed in the bacteria, this kana cassette can be excised and a knock out experiment is completed.
+This part includes a translational kanamycin resistance gene(in prokaryotes) or a neomycin resistance gene(in eukaryotes) and flanking loxP sites, which can be recognized by Cre recombinase. We can use DNA recombination technology to knock this part into E.coli genome and the antibiotic resistance gene helps us find the right colonies. Then if Cre is expressed in the bacteria, this kana cassette can be excised and a knock out experiment is completed.
 <!-- Add more about the biology of this part here