Difference between revisions of "Part:BBa K1587003"
Line 2: Line 2:
 
<partinfo>BBa_K1587003 short</partinfo>
 
<partinfo>BBa_K1587003 short</partinfo>
  
−
Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA.  
+
Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).  
  
 
<center>[[File:TLSE crt.jpg]]</center>
 
<center>[[File:TLSE crt.jpg]]</center>

Revision as of 15:29, 18 September 2015

3-hydroxybutyryl-CoA dehydratase

Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).

TLSE crt.jpg
Figure 1: Crotonyl-CoA formation catalyzed by Crt

We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).

TLSE ButMetabo.jpg
Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]