Difference between revisions of "Part:BBa K1797014"

Revision as of 15:27, 18 September 2015

Gam and Bet-Two Components of the Red Recombination System

Fig.1 The first step of gene editing using red recombination system (source: http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/red-swap.html)

Fig.2 The second step of gene editing using red recombination system (source: http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/red-swap.html)

Red recombination system is a efficient homologous recombination system found in lamda phage. It includes three main proteins, gam, bet and exo. Gam is able to bind RecBCD, inhibiting its ability to degrade exogenous linear DNA. Bet binds to the 3' overhang produced by exo and protects it, preventing its degradation by nucleases. Red recombination system is becoming popular in terms of gene editing. The common procedure is to PCR a certain fragment(often a kana cassette for easy screening later) flanked with homologous regions(about 20-50bp) first. Then the linear fragment and the red recombination system is introduced into bacteria for homologous recombination.

This part contains two components-gam and bet.

Sequence and Features