Difference between revisions of "Part:BBa K1157006:Experience"

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'''Conclusion:''' [https://parts.igem.org/Part:BBa_K082035 BBa_K082035] was ordered instead of cloned.
 
'''Conclusion:''' [https://parts.igem.org/Part:BBa_K082035 BBa_K082035] was ordered instead of cloned.
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<partinfo>BBa_K1157006 AddReview 2</partinfo>
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<I>iGEM_Stockholm_2015</I>
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'''Method:''' ''E. coli'' transformed with BBa_K1157006 were grown together with bacteria transformed with [https://parts.igem.org/Part:BBa_K082035 BBa_K082035] on normal agar plates without antibiotics.
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'''Results:''' The plates showed a slight red color change showed in Figure below.
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[[File:STHLM_readout_oneplate.png]]
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''Figure: Plate showing E. coli with BBa_K082035 spread in horizontal streaks E. coli with BBa_1157006 spread as an M on top. A slight red color change is seen, as expected.''
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'''Conclusion:''' Even though the two plates showed the correct color change, the results were however not reproducible at a later stage.
 
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Revision as of 15:26, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157006

We used flow cytometry to test the individual fluorescence expression of our biobrick from 0 to 4 hours and in different concentration(10-3, 10-5,10-7). Flow mCherry.jpg

User Reviews

UNIQ0f8f522b89cb7df6-partinfo-00000000-QINU

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iGEM_Stockholm_2015

Method: Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 E. coli using the transformation protocol and were supposed to be assembled using the 3A assembly protocol.

Results: During the initial lab weeks there were some trouble with transformation, later addressed to not using KCM buffer. The BBa_K082035 was ordered from IDT to speed up the process after repeated failed transformations and transformation troubleshooting were done in parallel.

Conclusion: BBa_K082035 was ordered instead of cloned.

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••

iGEM_Stockholm_2015

Method: E. coli transformed with BBa_K1157006 were grown together with bacteria transformed with BBa_K082035 on normal agar plates without antibiotics.

Results: The plates showed a slight red color change showed in Figure below.

STHLM readout oneplate.png Figure: Plate showing E. coli with BBa_K082035 spread in horizontal streaks E. coli with BBa_1157006 spread as an M on top. A slight red color change is seen, as expected.

Conclusion: Even though the two plates showed the correct color change, the results were however not reproducible at a later stage.

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