Difference between revisions of "Part:BBa K1587002"
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Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).
 
Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).
  
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<center>[[File:TLSE pcyA1.jpg]]</center>
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<center>[[File:TLSE pcyA ho1.jpg]]</center>
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<center>'''Figure 1: HOI-catalyzed heme degradation. (Ken ''et al'' 2009)'''</center>
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'''Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)'''
  
 
pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.
 
pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.
  
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<center>[[File:TLSE pcyA ho1.jpg]]</center>
+
 
−
'''Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)'''
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:25, 18 September 2015

pcyA with RBS

Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).

TLSE pcyA ho1.jpg

Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)

pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]