Difference between revisions of "Part:BBa K1587002"

Revision as of 15:24, 18 September 2015

pcyA with RBS

Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).

Figure 1: HOI-catalyzed heme degradation. (Ken et al 2009)

pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.

Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009) Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 790
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 343
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <center>'''Figure 1: HOI-catalyzed heme degradation. (Ken ''et al'' 2009)'''</center>
+pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.
+<center>[[File:TLSE pcyA ho1.jpg]]</center>
+'''Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)'''
 <!-- Add more about the biology of this part here
 ===Usage and Biology===