Difference between revisions of "Part:BBa K1587001"

Latest revision as of 15:22, 18 September 2015

acyl-CoA thioesterase 2

Figure 1 Butyrate formation catalyzed by tesB

The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

We used BBa_K1587001 with other genes to produce butyrate in Escherichia coli (Figure 2)

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 542
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 145
Illegal SapI.rc site found at 157

@@ Line 8: / Line 8: @@
 The integrity of our construct was checked by sequencing using VF2 primer.
-Used with other genes to produce butyrate in ''Escherichia coli'' (Figure 2)
+We used BBa_K1587001 with other genes to produce butyrate in ''Escherichia coli'' (Figure 2)
 <center>[[File:TLSE ButMetabo.jpg]]</center>