Difference between revisions of "Part:BBa K1633008:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements.
 
There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements.
  
−
This part is a codon-optimized nSMase 2 gene CDS. We express this gene in HEK 293 cell to increase the amount of exosomes.
+
This part is a codon-optimized nSMase 2 gene CDS. We express this gene in HEK293 cell to increase the amount of exosomes.
  
 
===Source===
 
===Source===

Latest revision as of 15:04, 18 September 2015


coding sequence to express nSMase 2 protein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 278
    Illegal BsaI.rc site found at 694
    Illegal BsaI.rc site found at 1600


Design Notes

There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements.

This part is a codon-optimized nSMase 2 gene CDS. We express this gene in HEK293 cell to increase the amount of exosomes.

Source

Homo Sapiens. The original sequence is from NCBI. NCBI Gene ID is 55512. The sequence codon of this part was optimized. We ordered the sequence from a DNA synthesis company


References