Difference between revisions of "Part:BBa K1597004"

Latest revision as of 14:38, 18 September 2015

slrR with strong promoter and RBS

SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing slrR, with the use of a strong promoter (BBa_K090504) and a strong RBS (BBa_K780001)(BBa_K780001), a robust biofilm can be formed. slrR with the promter and RBS was integrated via BBa_K823023 and via BBa_K1597001.

slrR mutant with an overexpression of slrR shows a bigger biofilm than the wildtype B. subtilis 3610 ΔcomI control. This phenotype could be due to the overexpression of the eps operon, leading to many extracellular polysaccharide substances. Resulting in a larger biofilm. The combination of the abrB knockout and slrR+ resulted in a biofilm size to the control. The process behind the this interaction is not known, however comparing these phenotypes clearly shows an interaction.

alt text

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1597004 short</partinfo>
-SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing SlrR, with the use of a strong promoter (BBa_K090504) and a strong RBS (BBa_K780001), a robust biofilm can be formed. ''slrR'' with the promter and RBS was integrated via K823023 and via K1597001
+SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing ''slrR'', with the use of a strong promoter <html><a href="https://parts.igem.org/Part:BBa_K090504"> (BBa_K090504)</a></html> and a strong RBS <html><a href="https://parts.igem.org/Part:BBa_K780001"> (BBa_K780001)</a></html>(BBa_K780001), a robust biofilm can be formed. ''slrR'' with the promter and RBS was integrated via <html><a href="https://parts.igem.org/Part:BBa_K823023"> BBa_K823023</a></html> and via <html><a href="https://parts.igem.org/Part:BBa_K1597001"> BBa_K1597001</a></html>.
-''slrR'' mutant with an overexpression of slrR has shows a bigger biofilm than the ''B. Subtilis comI'' control. This phenotype could be due to the overexpression of the eps operon, leading to many extracellular polysaccharide substances. Resulting in a larger biofilm.
+''slrR'' mutant with an overexpression of ''slrR'' shows a bigger biofilm than the wildtype ''B. subtilis 3610 ΔcomI'' control. This phenotype could be due to the overexpression of the eps operon, leading to many extracellular polysaccharide substances. Resulting in a larger biofilm.
-The combination of the abrB knockout and slrR resulted in a comparable biofilm to the control. This suggests that an abrB knockout together with slrR affect each other. The process behind the this interaction is not known, however comparing these phenotypes clearly shows an interaction.
+The combination of the abrB knockout and ''slrR''+ resulted in a biofilm size to the control. The process behind the this interaction is not known, however comparing these phenotypes clearly shows an interaction.