Difference between revisions of "Part:BBa K1603002:Design"
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===Source=== | ===Source=== | ||
| − | Genomic sequence from Saccharomyces cerevisiae CEN.PK2 | + | Genomic sequence from ''Saccharomyces cerevisiae'' CEN.PK2 |
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Latest revision as of 14:32, 18 September 2015
Promoter pTPI1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer: FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG
RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT
The primers were designed to remove the EcoRI site from the insert when constructing the biobrick to reduce the amount of base pairs in the primers. The primers also add 3 extra protective basepairs to the PstI site in the insert.
The construction of the final biobrick was initiated by cutting BBa_J04450 with XbaI, PstI, KnpI and FastAP and the insert with XbaI and PstI.
Source
Genomic sequence from Saccharomyces cerevisiae CEN.PK2