Difference between revisions of "Part:BBa K1583102"

 
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<partinfo>BBa_K1583102 short</partinfo>
 
<partinfo>BBa_K1583102 short</partinfo>
  
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This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655.
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The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations).
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Upon induction with rhamnose, CsgA is expressed and exported into the extracellular space. There, the membrane protein CsgB can trigger the CsgA protein monomers to aggregate and form amyloid nanofibres.
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CsgA with Hydroxyapatite-tag attachted to the C-terminus under control of L-rhamnose-inducible promoter ((<a href="https://parts.igem.org/Part:BBa_K914003"target="_blank">BBa_K914003</a>).
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===Usage and Biology===
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CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of <i>E.coli</i>. This protein is transported as an unfolded protein out of the cell. Outside the cell CsgA proteins self-assemble into nanowires after nucleation on the membrane protein CsgB. CsgC prevents CsgA proteins from self-assembling inside the cell and the transport is ensured by the proteins CsgEFG.
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This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of e.g. teeth. (Roy et al. 2008)
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1583102 parameters</partinfo>
 
<partinfo>BBa_K1583102 parameters</partinfo>
 
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Reference
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<p>Roy, M.D. et al., 2008. Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display. Advanced Materials, 20(10), pp.1830–1836.</p>

Revision as of 14:19, 18 September 2015

pRha + CsgA + Hydroxyapatite-affinity tag

CsgA with Hydroxyapatite-tag attachted to the C-terminus under control of L-rhamnose-inducible promoter ((BBa_K914003). CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli. This protein is transported as an unfolded protein out of the cell. Outside the cell CsgA proteins self-assemble into nanowires after nucleation on the membrane protein CsgB. CsgC prevents CsgA proteins from self-assembling inside the cell and the transport is ensured by the proteins CsgEFG. This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of e.g. teeth. (Roy et al. 2008) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Roy, M.D. et al., 2008. Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display. Advanced Materials, 20(10), pp.1830–1836.