Difference between revisions of "Part:BBa K1797009"
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| − | This part consists of a red light sensing system and an RFP reporter system. When bacterial colonies are exposed to red light, the OmpR promoter is not activated, preventing the expression of RFP. When colonies are kept in dark, the corresponding transcription factor of mpR promoter is activated and RFP is expressed, making the colonies red. | + | This part consists of a red light sensing system(BBa_K1797001) and an RFP reporter system. When bacterial colonies are exposed to red light, the OmpR promoter is not activated, preventing the expression of RFP. When colonies are kept in dark, the corresponding transcription factor of mpR promoter is activated and RFP is expressed, making the colonies red. |
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Revision as of 14:00, 18 September 2015
Red light sensing system with mRFP reporter
This part consists of a red light sensing system(BBa_K1797001) and an RFP reporter system. When bacterial colonies are exposed to red light, the OmpR promoter is not activated, preventing the expression of RFP. When colonies are kept in dark, the corresponding transcription factor of mpR promoter is activated and RFP is expressed, making the colonies red.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3871
Illegal XhoI site found at 425 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3424
Illegal AgeI site found at 4711
Illegal AgeI site found at 4823 - 1000COMPATIBLE WITH RFC[1000]