Difference between revisions of "Part:BBa K1583113"

Revision as of 13:39, 18 September 2015

pAra + fusion protein BFP_Spycatcher_His

This device expresses the fusion protein BFP_SpyCatcher_His under arabinose control.

It was created as a fusion protein from the following biobricks:

• Blue Fluorescent Protein BBa_K592100
  
• SpyCatcher BBa_K1583113
  
• His tag BBa_K1583059
  

CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms of E.coli. This protein is transported as an unfolded protein out of the cell. Outside the cell CsgA proteins self-assemble into nanowires after nucleation on the membrane protein CsgB. CsgC prevents CsgA proteins from self-assembling inside the cell and the transport is ensured by the proteins CsgEFG.

In our project we created Biobricks where we added specific tags to the CsgA protein in order to produce a functionalised amyloid nanowire. Examples are CsgA_His or the device CsgA_Hydroxyapatite affinity.