Difference between revisions of "Part:BBa K1603003:Design"

(→‎Design Notes)
(→‎Design Notes)
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Synthetic gene ordered from IDT.  
 
Synthetic gene ordered from IDT.  
  
−
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
+
Codon optimized for ''Saccharomyces cerevisiae'' and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
  
 
===Source===
 
===Source===

Revision as of 13:31, 18 September 2015


RecA - optimized for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1032


Design Notes

Synthetic gene ordered from IDT.

Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Source

Sequence adapted from Deinococcus Radiodurans. Ordered as synthetic gene.

References